rnascope multiplex fl v2 amp3 Search Results


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Expression of GFAP mRNA and comparison with MRI. ( A ) Representative T2 MRI image of a GB rat on PID8. A homogeneous hyperintense zone can be identified ipsilateral of the inoculation site. ( B ) Representative <t>RNAscope</t> image of the same GB rat on PID9. This image shows a large tumor (strong DAPI staining) surrounded by high level of GFAP mRNA expression. ( C ) Alignment of the hyperintense zone (red) and whole brain (yellow) on the T2 MRI image of a GB rat on PID8. Relative size of the hyperintense zone is calculated by dividing its area by the total area of the brain slice on MRI. ( D ) Alignment of tumor (blue) and tumor + rim of strong GFAP mRNA expression (red). Relative areas are calculated by dividing the blue and red area by the total area of the brain slice on the RNA scope image (yellow). ( E ) Representative T2 MRI image of a control rat (C4) on PID8. ( F ) Representative RNAscope image of C4 on PID9. The red arrow indicates the injection tract, in which there is an increase in GFAP mRNA expression.
Rnascope Multiplex Fl V2 Amp3, supplied by Advanced Cell Diagnostics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Expression of GFAP mRNA and comparison with MRI. ( A ) Representative T2 MRI image of a GB rat on PID8. A homogeneous hyperintense zone can be identified ipsilateral of the inoculation site. ( B ) Representative RNAscope image of the same GB rat on PID9. This image shows a large tumor (strong DAPI staining) surrounded by high level of GFAP mRNA expression. ( C ) Alignment of the hyperintense zone (red) and whole brain (yellow) on the T2 MRI image of a GB rat on PID8. Relative size of the hyperintense zone is calculated by dividing its area by the total area of the brain slice on MRI. ( D ) Alignment of tumor (blue) and tumor + rim of strong GFAP mRNA expression (red). Relative areas are calculated by dividing the blue and red area by the total area of the brain slice on the RNA scope image (yellow). ( E ) Representative T2 MRI image of a control rat (C4) on PID8. ( F ) Representative RNAscope image of C4 on PID9. The red arrow indicates the injection tract, in which there is an increase in GFAP mRNA expression.

Journal: International Journal of Molecular Sciences

Article Title: Development of a Rat Model for Glioma-Related Epilepsy

doi: 10.3390/ijms21196999

Figure Lengend Snippet: Expression of GFAP mRNA and comparison with MRI. ( A ) Representative T2 MRI image of a GB rat on PID8. A homogeneous hyperintense zone can be identified ipsilateral of the inoculation site. ( B ) Representative RNAscope image of the same GB rat on PID9. This image shows a large tumor (strong DAPI staining) surrounded by high level of GFAP mRNA expression. ( C ) Alignment of the hyperintense zone (red) and whole brain (yellow) on the T2 MRI image of a GB rat on PID8. Relative size of the hyperintense zone is calculated by dividing its area by the total area of the brain slice on MRI. ( D ) Alignment of tumor (blue) and tumor + rim of strong GFAP mRNA expression (red). Relative areas are calculated by dividing the blue and red area by the total area of the brain slice on the RNA scope image (yellow). ( E ) Representative T2 MRI image of a control rat (C4) on PID8. ( F ) Representative RNAscope image of C4 on PID9. The red arrow indicates the injection tract, in which there is an increase in GFAP mRNA expression.

Article Snippet: Then, RNAscope Multiplex FL V2 AMP2 (ACDbio, Newark, CA, USA), the amplifier, hybridizing the preamplifier to provide a support for the label probe and RNAscope Multiplex FL V2 AMP3 (ACDbio, Newark, CA, USA) serving as linker for the preamplifier and amplifier, were added for 30 and 15 min respectively at 40 °C.

Techniques: Expressing, Comparison, RNAscope, Staining, Slice Preparation, Control, Injection

MRI vs. RNAscope. This table summarizes the different relative areas calculated on MRI and RNAscope images. We can see that hyperintense areas on MRI extend beyond strongly DAPI-stained areas on RNAscope, but are smaller than areas including a high level of GFAP mRNA on RNAscope.

Journal: International Journal of Molecular Sciences

Article Title: Development of a Rat Model for Glioma-Related Epilepsy

doi: 10.3390/ijms21196999

Figure Lengend Snippet: MRI vs. RNAscope. This table summarizes the different relative areas calculated on MRI and RNAscope images. We can see that hyperintense areas on MRI extend beyond strongly DAPI-stained areas on RNAscope, but are smaller than areas including a high level of GFAP mRNA on RNAscope.

Article Snippet: Then, RNAscope Multiplex FL V2 AMP2 (ACDbio, Newark, CA, USA), the amplifier, hybridizing the preamplifier to provide a support for the label probe and RNAscope Multiplex FL V2 AMP3 (ACDbio, Newark, CA, USA) serving as linker for the preamplifier and amplifier, were added for 30 and 15 min respectively at 40 °C.

Techniques: RNAscope

Journal: Cell reports

Article Title: The adhesion GPCRs CELSR1–3 and LPHN3 engage G proteins via distinct activation mechanisms

doi: 10.1016/j.celrep.2023.112552

Figure Lengend Snippet:

Article Snippet: RNAscope Multiplex Fluorescent Assay: The following reagents were equilibrated at room temperature for 1 h: RNAscope Multiplex FL v2 AMP1 (Advanced Cell Diagnostics, 323101), RNAscope Multiplex FL v2 AMP2 (Advanced Cell Diagnostics, 323102), RNAscope Multiplex FL v2 AMP3 (Advanced Cell Diagnostics, 323103), RNAscope Multiplex FL v2 HRP C1 (Advanced Cell Diagnostics, 323104), RNAscope Multiplex FL v2 HRP C2 (Advanced Cell Diagnostics, 323105), RNAscope Multiplex FL v2 HRP C3 (Advanced Cell Diagnostics, 323106) and RNAscope Multiplex FL v2 HRP Blocker (Advanced Cell Diagnostics, 323107).

Techniques: Virus, Recombinant, Membrane, Electron Microscopy, Clone Assay, Software

Journal: Cell reports

Article Title: The adhesion GPCRs CELSR1–3 and LPHN3 engage G proteins via distinct activation mechanisms

doi: 10.1016/j.celrep.2023.112552

Figure Lengend Snippet:

Article Snippet: RNAscope Multiplex Fluorescent Assay: The following reagents were equilibrated at room temperature for 1 h: RNAscope Multiplex FL v2 AMP1 (Advanced Cell Diagnostics, 323101), RNAscope Multiplex FL v2 AMP2 (Advanced Cell Diagnostics, 323102), RNAscope Multiplex FL v2 AMP3 (Advanced Cell Diagnostics, 323103), RNAscope Multiplex FL v2 HRP C1 (Advanced Cell Diagnostics, 323104), RNAscope Multiplex FL v2 HRP C2 (Advanced Cell Diagnostics, 323105), RNAscope Multiplex FL v2 HRP C3 (Advanced Cell Diagnostics, 323106) and RNAscope Multiplex FL v2 HRP Blocker (Advanced Cell Diagnostics, 323107).

Techniques: Virus, Recombinant, Membrane, Electron Microscopy, Clone Assay, Software